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| Registros recuperados : 26 | |
| 2. |  | PERES, R. F. G.; CABRAL, D. D.; TETZNER, T. A. D. Avaliação terapêutica das bases farmacológicas: abamectin, doramectin, ivermectin e moxidectin em ovinos a nível de campo em Uberlândia, MG. In: CONGRESSO BRASILEIRO DE MEDICINA VETERINÁRIA, 31., 2004, São luis. A medicina veterinária no novo milênio: transformação social, preservação ambiental e segurança alimentar: resumos. São Luis, Sociedade Brasileira de Medicina Veterinária, 2004. Seção produção animal e agronegócio. 1 CD-ROM.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 8. | | SARAIVA, N. Z.; PERECIN, F.; MÉO, S. C.; FERREIRA, C. R.; TETZNER, T. A. D.; GARCIA, J. M. Demecolcine effects on microtubule kinetics and chemically assisted enucleation of bovine oocytes. Cloning and Stem Cells, v. 11, n. 1, p. 141-152, mar. 2009.| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 9. | | SARAIVA, N. Z.; PERECIN, F.; MÉO, S. C.; FERREIRA, C. R.; TETZNER, T. A. D.; VANTINI, R.; GARCIA, J. M. Efeitos da demecolcina sobre a cinética da maturação nuclear e a migração dos grânulos corticais em oócitos bovinos. Acta Scientiae Veterinariae, v. 35, supl. 3, p. s1276, 2007; In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE TECNOLOGIA DE EMBRIÕES, 21., 2007, Salvador. Anais... Salvador: UFRGS, 2007. p. 1276.| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 10. | | TETZNER, T. A. D.; SARAIVA, N. Z.; PERECIN, F.; NICIURA, S. C. M.; FERREIRA, C. R.; OLIVEIRA, C. S.; GARCIA, J. M. The effects of ovalbumin as a protein source during the in vitro production of bovine embryos. Revista Brasileira de Zootecnia, v. 40, n. 10, p. 2135-2141, oct. 2011.| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 11. | | TETZNER, T. A. D.; SARAIVA, N. Z.; OLIVEIRA, C. S.; NICIURA, S. C. M.; SOUZA M. M.; LIMA, M. R.; GARCIA J. M. Effects of culture with ovalbumin in absence of fetal bovine serum and bovine serum albumin on in vitro production of cattle embryos. In: ANNUAL CONFERENCE OT THE INTERNATIONAL EMBRYO TRANSFER SOCIETY, 36., 2010, Cordoba. Proceedings... Córdoba: ITES, 2010; Reproduction, Fertility and development, v. 22, n. 1, p. 197, 2010.| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 12. | | SARAIVA, N. Z.; OLIVEIRA, C. S.; TETZNER, T. A. D.; LIMA, M. R. de; NICIURA, S. C. M.; GARCIA, J. M. Effects of demecolcine on the meiotic cell-cycle and microtubular kinetics of activated bovine oocytes submitted to chemical enucleation. In: ANUAL CONFERENCE OF INTERNATIONAL EMBRYO TRANSFER SOCIETY, 24., Phoenix, Arizona. Phoenix: CSIRO, 2012 p. 119| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 13. | | SARAIVA, N. Z.; PERECIN, F.; MÉO, S. C.; FERREIRA, C. R.; TETZNER, T. A. D.; OLIVEIRA, J. C.; GARCIA, J. M. Effects of demecolcine on microtubule composition and chemically assisted enucleation of bovine oocytes. Reproduction, Fertility and Development, v. 20, n. 1, p. 107-108, 2008.| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 14. | | TETZNER, T. A. D.; SARAIVA, N. Z.; PERECIN, F.; OLIVEIRA, C. S.; MONTEIRO, F. M.; LIMA, M. R.; NICIURA, S. C. M.; FERREIRA, C. R.; GARCIA, J. M. Effects of embryonic fluid and serum replacer as protein sources for in vitro maturation of bovine oocytes. In: INTERNATIONAL SYMPOSIUM ANIMAL BIOLOGY OF REPRODUCTIVE, 3., 2010, Águas de São Pedro: CBRA: USP/FMVZ, 2010| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 15. | | SARAIVA, N. Z.; OLIVEIRA, C. S.; TETZNER, T. A. D.; LIMA, M. R. de; NICIURA, S. C. M.; GARCIA, J. M. Expressão dos genes xist, g6pd e hspa1 em blastocistos bovinos reconstituídos por tn a partir de oócitos receptores produzidos por enucleação assistida quimicamente. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE TECNOLOGIA DE EMBRIÕES, 25., 2011, Cumbuco. O impacto das biotecnologias reprodutivas na saúde e produção animal - anais. Cumbuco: SBTE, 2011. p. 437| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 16. | | SARAIVA, N. Z.; OLIVEIRA, C. S.; TETZNER, T. A. D.; LIMA, M. R. de; MELO, D. S. de; NICIURA, S. C. M.; GARCIA, J. M. Chemically assisted enucleation results in higher G6PD expression in early bovine female embryos obtained by somatic cell nuclear transfer. Cellular Reprogramming, v. 14, n. 5, p. 1-11, 2012.| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 17. | | SARAIVA, N. Z.; OLIVEIRA, C. S.; TETZNER, T. A. D.; SOUZA, M. M.; LIMA, M. R. de; NICIURA, S. C. M.; GARCIA, J. M. Bovine cytoplasts prepared by demecolcine-induced enucleation of activated oocytes. In: ANNUAL CONFERENCE OT THE INTERNATIONAL EMBRYO TRANSFER SOCIETY, 36., 2010, Cordoba. Proceedings... Córdoba: ITES, 2010; Reproduction, Fertility and development, v. 22, n. 1, 2010. p. 197| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 18. | | MEO, S. C.; FERREIRA, C. R.; PERECIN, F.; SARAIVA, N. Z.; TETZNER, T. A. D.; YAMAZAKI, W.; LEA, C. L. V.; MEIRELLES, F. V.; GARCIA, J. M. Karyoplast exchange between strontium- and 6-DMAP-parthenogenetically activated zygotes of cattle. Animal Reproduction Science, v. 116, n. 3-4, p. 381-385, dec. 2009.| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 19. | | SARAIVA, N. Z.; OLIVEIRA, C. S.; TETZNER, T. A. D.; SOUZA, M. M.; LIMA, M. R.; NICIURA, S. C. M.; GARCIA, J. M. Métodos alternativos de enucleação oocitária utilizados na transferência nuclear em animais. Revista Brasileira de Reprodução Animal, v. 34, n. 4, p. 197-205, out./dez. 2010.| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 20. | | TETZNER, T. A. D.; SARAIVA, N. Z.; PERECIN, F.; FERREIRA, C. R.; MÉO, S. C.; OLIVEIRA, C. S.; MELO, D. S.; MONTEIRO, F. M.; VANTINI, R.; GARCIA, J. M. Efeitos da substituição do soro fetal bovino (SFB) e da albumina sérica bovina (BSA) pela ovalbumina (OVA) na produção in vitro de embriões bovinos. Acta Scientiae Veterinariae, v. 35, supl. 3, p. s1181, 2007; In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE TECNOLOGIA DE EMBRIÕES, 21., 2007, Salvador. Anais... Salvador: UFRGS, 2007.| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| Registros recuperados : 26 | |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Pecuária Sudeste. Para informações adicionais entre em contato com cppse.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Pecuária Sudeste. |
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Data corrente: |
25/05/2009 |
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Data da última atualização: |
15/03/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
SARAIVA, N. Z.; PERECIN, F.; MÉO, S. C.; FERREIRA, C. R.; TETZNER, T. A. D.; GARCIA, J. M. |
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Afiliação: |
NAIARA ZOCCAL SARAIVA, UNESP/JABOTICABAL; FELIPE PERECIN, FZEA/USP; SIMONE CRISTINA MEO NICIURA, CPPSE; CHRISTINA RAMIRES FERREIRA, FZEA/USP; TATIANA ALMEIDA DRUMOND TETZNER, UNESP/JABOTICABAL; JOAQUIM MANSANO GARCIA, UNESP/JABOTICABAL. |
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Título: |
Demecolcine effects on microtubule kinetics and chemically assisted enucleation of bovine oocytes. |
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Ano de publicação: |
2009 |
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Fonte/Imprenta: |
Cloning and Stem Cells, v. 11, n. 1, p. 141-152, mar. 2009. |
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DOI: |
10.1089/clo.2008.0044 |
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Idioma: |
Inglês |
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Conteúdo: |
This study aimed to evaluate the effect of demecolcine, a microtubule-depolymerizing agent, on microtubule kinetics; to determine the best concentration of demecolcine as a chemically assisted enucleation agent in metaphase I (MI) and metaphase II (MII) bovine oocytes, and to evaluate the embryonic development after nuclear transfer (NT) using chemically assisted enucleation of recipient oocytes. Oocytes in vitro matured for 12 h (MI) and 21 h (MII) were exposed to several concentrations of demecolcine and evaluated for enucleation or membrane protrusion formation. Demecolcine concentration of 0.05 microg/mL produced the highest rates of enucleation in group MI (15.2%) and protrusion formation in group MII (55.1%), and was employed in the following experiments. Demecolcine effect was seen as early as 0.5 h after treatment, with a significant increase in the frequency of oocytes with complete microtubule depletion in MI (58.9%) and MII (21.8%) compared to initial averages at 0 h (27.4% and 1.9%, respectively). Microtubule repolymerization was observed when MII-treated oocytes were cultured in demecolcine-free medium for 6 h (42.4% oocytes with two evident sets of microtubules). Chemically assisted enucleated oocytes were used as recipient cytoplasts in NT procedures to assess embryonic development. For NT, 219 of 515 oocytes (42.5%) formed protrusions and were enucleated, and reconstructed, resulting in 58 nuclear-transferred one-cell embryos. Cleavage (84.5%) and blastocyst development (27.6%) rates were assessed. In conclusion, demecolcine can be used at lower concentrations than routinely employed, and the chemically assisted enucleation technique was proven to be highly efficient allowing embryonic development in bovine. MenosThis study aimed to evaluate the effect of demecolcine, a microtubule-depolymerizing agent, on microtubule kinetics; to determine the best concentration of demecolcine as a chemically assisted enucleation agent in metaphase I (MI) and metaphase II (MII) bovine oocytes, and to evaluate the embryonic development after nuclear transfer (NT) using chemically assisted enucleation of recipient oocytes. Oocytes in vitro matured for 12 h (MI) and 21 h (MII) were exposed to several concentrations of demecolcine and evaluated for enucleation or membrane protrusion formation. Demecolcine concentration of 0.05 microg/mL produced the highest rates of enucleation in group MI (15.2%) and protrusion formation in group MII (55.1%), and was employed in the following experiments. Demecolcine effect was seen as early as 0.5 h after treatment, with a significant increase in the frequency of oocytes with complete microtubule depletion in MI (58.9%) and MII (21.8%) compared to initial averages at 0 h (27.4% and 1.9%, respectively). Microtubule repolymerization was observed when MII-treated oocytes were cultured in demecolcine-free medium for 6 h (42.4% oocytes with two evident sets of microtubules). Chemically assisted enucleated oocytes were used as recipient cytoplasts in NT procedures to assess embryonic development. For NT, 219 of 515 oocytes (42.5%) formed protrusions and were enucleated, and reconstructed, resulting in 58 nuclear-transferred one-cell embryos. Cleavage (84.5%) and blastocyst ... Mostrar Tudo |
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Thesaurus NAL: |
oocytes. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
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001 1048984
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024 7 $a10.1089/clo.2008.0044$2DOI
100 1 $aSARAIVA, N. Z.
245 $aDemecolcine effects on microtubule kinetics and chemically assisted enucleation of bovine oocytes.$h[electronic resource]
260 $c2009
520 $aThis study aimed to evaluate the effect of demecolcine, a microtubule-depolymerizing agent, on microtubule kinetics; to determine the best concentration of demecolcine as a chemically assisted enucleation agent in metaphase I (MI) and metaphase II (MII) bovine oocytes, and to evaluate the embryonic development after nuclear transfer (NT) using chemically assisted enucleation of recipient oocytes. Oocytes in vitro matured for 12 h (MI) and 21 h (MII) were exposed to several concentrations of demecolcine and evaluated for enucleation or membrane protrusion formation. Demecolcine concentration of 0.05 microg/mL produced the highest rates of enucleation in group MI (15.2%) and protrusion formation in group MII (55.1%), and was employed in the following experiments. Demecolcine effect was seen as early as 0.5 h after treatment, with a significant increase in the frequency of oocytes with complete microtubule depletion in MI (58.9%) and MII (21.8%) compared to initial averages at 0 h (27.4% and 1.9%, respectively). Microtubule repolymerization was observed when MII-treated oocytes were cultured in demecolcine-free medium for 6 h (42.4% oocytes with two evident sets of microtubules). Chemically assisted enucleated oocytes were used as recipient cytoplasts in NT procedures to assess embryonic development. For NT, 219 of 515 oocytes (42.5%) formed protrusions and were enucleated, and reconstructed, resulting in 58 nuclear-transferred one-cell embryos. Cleavage (84.5%) and blastocyst development (27.6%) rates were assessed. In conclusion, demecolcine can be used at lower concentrations than routinely employed, and the chemically assisted enucleation technique was proven to be highly efficient allowing embryonic development in bovine.
650 $aoocytes
700 1 $aPERECIN, F.
700 1 $aMÉO, S. C.
700 1 $aFERREIRA, C. R.
700 1 $aTETZNER, T. A. D.
700 1 $aGARCIA, J. M.
773 $tCloning and Stem Cells$gv. 11, n. 1, p. 141-152, mar. 2009.
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